![]() ![]() HALO-5 HPLC columns are made using ground- breaking Fused-Core particle technology (core-shell particles) to. Monodispersed microspheres are always preferred as packing materials. greater resolving power for complex samples. The LbL approach is the main method for the preparation of such particles. Next, optimizing the pore size and shell thickness for intact proteins or smaller peptide fragments provides well-defined depth penetration of biomolecules leading to maximum separation power. Coreshell (or fused-core) silica microspheres are mostly used as the packing materials for HPLC columns. Phenomenex HPLC scientists and staff provide an inside look into the making and development of Kinetex core-shell HPLC column technology for reversed phase chromatography. The Phenyl-Hexyl phase is recommended for complex samples where some peaks are resolved on classical RP phases but not on a conventional phenyl phase and at the same time other peaks are resolved on a phenyl phase but not well on a conventional alkyl phase.Core-Shell Particles Precision Engineered for Protein and Peptide Separations.Ĭore-shell particle technology provides striking increases in peak capacity and resolution at lower backpressure, giving chromatographers the ability to achieve ultra-high performance on ANY system, HPLC or UHPLC.Ī uniform porous silica layer is grown around a solid, spherical silica core, providing effective retention and selectivity with improved resolution, speed, and recovery. The first commercially available sorbent in this new generation of core-shell particles was the Halo from Advanced Material Technologies (Wilmington, DE). Restek is proud to announce that SPP core-shell technology has evolved with. Moreover, fused-core and core-shell technology columns are ideal for. BlueShell application areasĬ6 chain offers classical RP retention and selectivity, the phenyl ring adds special interaction with polar groups. Increased sample throughput with existing HPLC and UHPLC instrumentation. 5.4 LIQUID CHROMATOGRAPHY LC-MS method is one of the most promising approaches for. We use cookies to improve your experience and our website service. The reduced depth of the outer porous layer limits the diffusional path of analytes, leading to minimized mass transfer resistance and minimized peak broadening. Kinetex core-shell technology columns are designed to help you improve your column performance, sensitivity, and efficiency across an platform and particle size. Evolution of polymer coating technology in core-shell CAPCELL. Fortis Technologies Ltd, 45 Coalbrookdale Road, Cheshire, CH64 3UG, UK. CAPCELL CORE provides high-speed and improved separation in UHPLC as well as conventional HPLC. The 2.6 µm diameter particles enable high speed and high resolution separations without undesirable excessive backpressure. Strength in Technology New Core Shell Technology Comparison of New Core-Shell Technology Ken Butchart, Mark Woodruff. ![]() Take advantage of core-shell particle technologyĬompared to classical fully porous silica materials, BlueShell particles consist of a solid core and a porous shell. Significantly lower backpressures than sub-2 μm particles Multiple columns can be combined for even higher resolutionĮfficiency is comparable with fully porous sub-2 μm particles Highest separating power (~ 200.000 theoretical plates/meter) To learn more about Fortis SpeedCore, check out the links below. BlueShell UHPLC columns Vertex Plus column BlueShell Phenyl-Hexyl core-shellīueShell columns offer ultra high performance without ultra high pressure: packed with core-shell particles, they provide improved speed, higher resolution, and reduced eluent consumption with moderate HPLC backpressure. Fortis SpeedCore (core-shell particles) are compatible with both HPLC and UHPLC systems.
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